CD4 Mouse Monoclonal Antibody [Clone ID: RPA-T4]

CAT#: SM1058LE

CD4 mouse monoclonal antibody, clone RPA-T4, Low Endotoxin

Specifications

Product Data

• Clone Name: RPA-T4
• Applications: FC, FN, IHC
• Recommended Dilution: Functional Assays.
  Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10⁶ cells or 100 µl whole blood.
  Immunohistochemistry on Frozen Sections: 1/200-1/2000.
  The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections.
  Recommended Positive Control: Human lymphatic tissue.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises CD4. Epitope mapping studies have shown that clone RPA-T4 recognises an epitope within domain 1 of the extracellular region of the CD4 molecule.
  Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation (See Voehringer, D. et al. for details).
• Formulation: PBS, pH 7.4
  State: Low Endotoxin
  State: Liquid purified IgG fraction
  Stabilizer: None
  Preservative: None
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Database Link:
  Entrez Gene 920 Human
• Background: CD4 is a 55kD cell surface glycoprotein that is expressed primarily on a subpopulation of T lymphocytes and on peripheral blood monocytes and tissue macrophages.
• Synonyms: T-cell surface antigen T4/Leu-3