CD4 Mouse Monoclonal Antibody [Clone ID: RPA-T4]

CAT#: SM1058P

CD4 mouse monoclonal antibody, clone RPA-T4, Purified

Product Images

Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4

Specifications

Product Data

• Clone Name: RPA-T4
• Applications: FC, IHC
• Recommended Dilution: Flow cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells or 100µl whole blood.
  Immunohistochemistry on frozen sections: 1/10 - 1/100.
  Recommended positive control: Human lymphatic tissue.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises CD4. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognise an epitope within domain 1, of the extracellular region, of the CD4 molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation. We recommend the use of SM1058LE for this purpose.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography on Protein G
• Database Link:
  Entrez Gene 920 Human
• Background: CD4 is a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages.
• Synonyms: T-cell surface antigen T4/Leu-3