CD9 Mouse Monoclonal Antibody [Clone ID: MM2/57]
Specifications
Product Data | |
Clone Name | MM2/57 |
Applications | FC |
Recommended Dilution | Flow Cytometry: Use 10 µl of Neat-1/2 diluted antibody to label 10e6 cells or 100 µl whole blood. |
Reactivities | Bovine, Canine, Equine, Feline, Human, Porcine, Rabbit |
Host | Mouse |
Isotype | IgG2b |
Clonality | Monoclonal |
Immunogen | Human platelet membrane. Spleen cells from immunised BALB/c mice were fused with cells from the SP2/0 mouse myeloma line. |
Specificity | This antibody is a 24kD cell surface glycoprotein expressed by platelets, monocytes, some lymphocytes and endothelial cells. It is a member of the tetraspans gene family. |
Formulation | PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: PE State: Lyophilized purified IgG fraction. Label: R. Phycoerythrin (RPE) |
Reconstitution Method | Restore with distilled water. |
Purification | Affinity chromatography on Protein A |
Conjugation | PE |
Storage | Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve. |
Synonyms | MIC3, TSPAN29, GIG2, p24, Tetraspanin-29, 5H9 antigen |
Reference Data |
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