CD11b (ITGAM) Mouse Monoclonal Antibody [Clone ID: ICRF44]

CAT#: SM1068P

CD11b (ITGAM) mouse monoclonal antibody, clone ICRF44, Purified

Product Images

Staining of human peripheral blood granulocytes with Mouse anti Human CD11b.

Specifications

Product Data

• Clone Name: ICRF44
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/10-1/100 diluted antibody to label 10⁶ cells or 100 µl whole blood.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections: 1/100-1/1000.
  The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections.
  Recommended Positive Control: Human tonsil.
  Not suitable for Paraffin Embedded Sections.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rheumatoid synovial cells and Human monocytes.
• Specificity: This antibody recognizes the CD11b cell surface glycoprotein.
  Clone ICRF44 has been reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha M
• Database Link:
  Entrez Gene 3684 Human
• Background: CD11b is a 165 kD molecule also know as the alpha-M integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta-2 integrin and is found on monocytes, granulocytes, NK cells and some peripheral blood lymphocytes. ICR44 significantly blocks homotypic PNM aggregation on response to FMLP (3,4).
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1