CD14 Mouse Monoclonal Antibody [Clone ID: UCHM1]

CAT#: SM1071APCT

CD14 mouse monoclonal antibody, clone UCHM1, APC

Product Images

Staining of human peripheral blood monocytes with Mouse Anti Human CD14-APC

Specifications

Product Data

• Clone Name: UCHM1
• Applications: FC
• Recommended Dilution: Flow Cytometry.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human Thymocytes followed by peripheral blood mononuclear cells. Spleen cells from an immunised mouse were fused with cells of a mouse myeloma line.
• Specificity: This antibody recognises the CD14 cell surface antigen, a 55kD glycoprotein expressed by monocytes and macrophages.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% Bovine Serum Albumin as stabilizer.
  Label: APC
  State: Lyophilized purified IgG fraction.
  Label: Allophycocyanin
• Reconstitution Method: Restore with 1 ml distilled water
• Purification: Affinity chromatography on Protein G
• Conjugation: APC
• Storage: Prior to and following reconstitution store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD14 molecule
• Database Link:
  Entrez Gene 929 Human
• Background: CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.
• Synonyms: CD14