CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: 3G8]

CAT#: SM1072LE

CD16 (FCGR3A) mouse monoclonal antibody, clone 3G8, Low Endotoxin

Product Images

Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE-CyTM7.

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  Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) PE.

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  Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) APC-CyTM7.

Specifications

Product Data

• Clone Name: 3G8
• Applications: FC, FN, IHC, IP
• Recommended Dilution:
  Flow cytometry: 6 μg/ml.
  Immunoprecipitation.
  Immunohistochemistry (frozen sections): acetone fixation.
  Functional application: In vitro Stimulation of NK cell proliferation, blocking of IgG binding and phagocytosis, inhibition of cytotoxic activity, in vivo NK cell depletion.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human neutrophils
• Specificity: This antibody recognizes CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).
• Formulation: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
  State: Low Endotoxin
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2214 Human
• Background: CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
• Synonyms: FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2