CD18 (ITGB2) Rat Monoclonal Antibody [Clone ID: YFC118.3]

CAT#: SM1076R

CD18 (ITGB2) rat monoclonal antibody, clone YFC118.3, PE

Product Images

Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD18:RPE

Specifications

Product Data

• Clone Name: YFC118.3
• Applications: FC
• Recommended Dilution: Flow cytometry: Use 10 µl of neat antibody to label 100 µl whole blood or 10e6 cells in 100 µl.
• Reactivities: Canine, Human
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the Y3/Ag.1.2.3 rat myeloma cell line.
• Specificity: YFC118.3 was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigen as recognising the CD18 antigen. The main cellular reactivity of antibody YFC 118.3 is a strong reactivity with Leucocytes (platelets negative). YFC118.3 blocks homotypic adhesion and other lymphocyte functions which involve the LFA-1 antigen.
• Formulation: PBS, pH7.2 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: PE
  State: Lyophilized purified IgG
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: SM1076R: Restore with 1 ml distilled water.
  SM1076RT: Restore with 25 µl distilled water.
• Purification: Affinity chromatography on Protein G
• Conjugation: PE
• Storage: Prior to and following reconstitution store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3689 Human
• Background: The CD18 antigen is an integral membrane glycoprotein of 95kD molecular weight - known as the b chain, of the LFA-1 complex. The CD18 antigen is non-covalently linked to CD11a, b, c molecules. The CD18 molecule is the receptor for ICAM-1 and is thus important for cell adhesion.
• Synonyms: Integrin beta-2, MFI7