CD27 Mouse Monoclonal Antibody [Clone ID: LT27]

CAT#: SM1086APC

CD27 mouse monoclonal antibody, clone LT27, APC

Product Images

Surface staining of human peripheral blood cells with anti-human CD27 (LT27) APC.

Specifications

Product Data

• Clone Name: LT27
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human peripheral blood lymphocytes
• Specificity: This antibody reacts with CD27 (T14), a 50-55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells.
• Formulation: Phosphate buffered saline (PBS) solution containing 15mM sodium azide
  Label: APC
  State: Liquid Ig fraction
  Label: Conjugated with cross-linked Allophycocyanin under optimum conditions
• Purification: Size-exclusion chromatography
• Conjugation: APC
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 939 Human
• Background: CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70.
• Synonyms: TNFRSF7, CD27L receptor, T14