CD28 Rat Monoclonal Antibody [Clone ID: YTH913.12]

CAT#: SM1087R

CD28 rat monoclonal antibody, clone YTH913.12, PE


USD 495.00

5 Days*

Size
    • 100 Tests

Product Images

Specifications

Product Data
Clone Name YTH913.12
Applications FC
Recommended Dilution Flow Cytometry.
Reactivities Human
Host Rat
Isotype IgG2b
Clonality Monoclonal
Immunogen Human peripheral blood T-cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
Specificity This antibody recognises the human CD28 cell surface antigen, a 44kD glycoprotein expressed by a subset of T lymphocytes.
It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.
Formulation PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Label: PE
State: Lyophized purified IgG fraction.
Label: R. Phycoerythrin (RPE)
Reconstitution Method Restore with distilled water
Purification Affinity chromatography on Protein G.
Conjugation PE
Storage Prior to and following reconstitution store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Stability Shelf life: one year from despatch.
Background Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.
Synonyms TP44
Reference Data

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