CD28 Rat Monoclonal Antibody [Clone ID: YTH913.12]

CAT#: SM1087R

CD28 rat monoclonal antibody, clone YTH913.12, PE

Product Images

Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28:RPE (SM1087R).

Specifications

Product Data

• Clone Name: YTH913.12
• Applications: FC
• Recommended Dilution: Flow Cytometry.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human peripheral blood T-cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
• Specificity: This antibody recognises the human CD28 cell surface antigen, a 44kD glycoprotein expressed by a subset of T lymphocytes.
  It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: PE
  State: Lyophized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with distilled water
• Purification: Affinity chromatography on Protein G.
• Conjugation: PE
• Storage: Prior to and following reconstitution store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 940 Human
• Background: Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.
• Synonyms: TP44