CD15 Mouse Monoclonal Antibody [Clone ID: TG-1]

CAT#: SM1089B

CD15 mouse monoclonal antibody, clone TG-1, Biotin

Specifications

Product Data

• Clone Name: TG-1
• Applications: FC
• Recommended Dilution: In flow cytometry (1.0 x 10e6 cells ), IFA and IHC (acetone-fixed frozen sections). May be used for enumerating mature granulocytes in peripheral blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Thymocyte glycoprotein fraction adsorbed to Con A and eluted with 0.1 M glucose
• Specificity: Recognizes a (Mr 156 kDa) cell surface glycoprotein expressed on virtually all peripheral blood granulocytes.(1,2,3). Anti-CD15 has virtually no reactivity with T or B cells.(1,2,3).
• Formulation: 0.01 M PBS, pH7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium azide as preservative
  Label: Biotin
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ion exchange chromatography and gel filtration
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Should this product contain a precipitate we recommend microcentrifugation before use.
• Stability: Shelf life: one year from despatch.
• Background: The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.
• Synonyms: Lewis X, X-Hapten, Lacto-N-Fucopentaose III, Stage-Specific Embryonic Antigen, SSEA1