CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: AT10]
Specifications
Product Data | |
Clone Name | AT10 |
Applications | FC |
Recommended Dilution | Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells or 100 µl whole blood. |
Reactivities | Canine, Human, Monkey |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | K562 cell line. |
Specificity | The antibody recognizes the Human CD32 antigen. Clone AT10 blocks the binding of IgG to Fc gamma R11 (See Ref. 4). The use of SM1092LE is recommended for this purpose. |
Formulation | PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1 |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Conjugation | FITC |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | Human CD32 antigen is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. |
Synonyms | Fc-gamma RII-a, Fc-gamma-RIIa, CDw32, FCGR2A, FCG2, FCGR2A1, IGFR2 |
Reference Data |
Documents
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