CD35 (CR1) Mouse Monoclonal Antibody [Clone ID: E11]

CAT#: SM1097AS

CD35 (CR1) mouse monoclonal antibody, clone E11, Azide Free

Product Images

Formalin-Fixed, Paraffin-Embedded Human Kidney stained with Azide Free CR1 Antibody (Clone E11) 10 ug/ml after heat-induced antigen retrieval.

Specifications

Product Data

• Clone Name: E11
• Applications: FC, IHC, WB
• Recommended Dilution: Flow Cytometry: 1/50 - 1/100.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot.
• Reactivities: Human, Monkey, Baboon
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human acute monocytic leukaemia cells.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody is a single chain cell surface glycoprotein which exists in four allotypic forms (A,B,C,D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement.
• Formulation: PBS, pH 7.4
  State: Azide Free
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: None
  Preservative: None
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens complement component 3b/4b receptor 1 (Knops blood group) (CR1), transcript variant F
• Database Link:
  Entrez Gene 718120 MonkeyEntrez Gene 1378 Human
• Background: CD35 is a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.
• Synonyms: Complement receptor type 1, CR1, C3b/C4b receptor

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Hematopoietic cell lineage