CD33 Mouse Monoclonal Antibody [Clone ID: WM53]
Specifications
Product Data | |
Clone Name | WM53 |
Applications | FC |
Recommended Dilution | Flow cytometry. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Human AML cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line. |
Specificity | This antibody recognises the CD33 cell surface glycoprotein. |
Formulation | PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer 1 |
Concentration | lot specific |
Purification | Affinity chromatography on Protein G |
Conjugation | FITC |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. |
Stability | Shelf life: one year from despatch. |
Gene Name | CD33 molecule |
Database Link | |
Background | CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67kDa M.W. surface antigen. CD33 is a 67kD molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias. |
Synonyms | Siglec-3, gp67 |
Reference Data |
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