CD45 (PTPRC) (CD45RA) Mouse Monoclonal Antibody [Clone ID: MEM-56]

CAT#: SM1115R

CD45 (PTPRC) (CD45RA) mouse monoclonal antibody, clone MEM-56, PE

Specifications

Product Data

• Clone Name: MEM-56
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10e6 cells in a suspension.
  The content of vial (2 ml) is sufficient for 100 Tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human Thymocytes and T Lymphocytes.
• Specificity: The antibody reacts with CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.
• Formulation: PBS containing 15 mM Sodium Azide as preservative and 0.2% (w/v) high-grade protease free BSA as a stabilizing agent.
  Label: PE
  State: Liquid purified Ig fraction
  Label: Conjugated with R-Phycoerythrin under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use
• Conjugation: PE
• Storage: Store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on nave T cells and normal plasma cells.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200