CD45 (PTPRC) (CD45RO) Mouse Monoclonal Antibody [Clone ID: UCHL1]

CAT#: SM1117PS

CD45 (PTPRC) (CD45RO) mouse monoclonal antibody, clone UCHL1, Purified

Product Images

Staining of human peripheral blood lymphocytes with Mouse Anti Human CD45RO.

Specifications

Product Data

• Clone Name: UCHL1
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/25-1/50 diluted antibody to label 10e6 cells in 100 µl.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  PImmunohistochemistry on Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin sections and does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
  Recommended Positive Control Tissue: Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human IL-2 dependent T-Cells.
• Specificity: This antibody recognises the low molecular weight isoform (180kD) of the Leucocyte Common Antigen (LCA).
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch
• Database Link:
  Entrez Gene 5788 Human
• Background: The antigen is expressed by a functional subset of T cells with memory phenotype. In peripheral blood UCHL1 stains 40-80% of lymphocytes, and all monocytes and granulocytes.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200