CD44 Mouse Monoclonal Antibody [Clone ID: VFF-7]

CAT#: SM1118F

CD44 mouse monoclonal antibody, clone VFF-7, FITC

Specifications

Product Data

• Clone Name: VFF-7
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody solution to label 10e6 cells in 100 µl.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of Human CD44.
  Spleen cells from immunised BALB/c micewere fused with cells of the P3X63Ag8.653 myeloma cell line.
• Specificity: This antibody specifically recognises an epitope encoded by exon v6 on the variant portion of Human CD44.
• Formulation: Tris buffered saline containing 0.02% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch
• Database Link:
  Entrez Gene 960 Human
• Background: CD44 is the receptor for hyaluronic acid (HA). It mediates cell to cell and cell matrix interactions through its affinity for HA. Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. High protein heterogeneity due to several alternative splicing and post translational modification events. CD44R represents a heterogeneous group of CD44 variant isoforms expressed constitutively in epithelial cells and monocyte lineage cells and can be upregulated in activated leukocytes. CD44R, and in particular CD44v6 containing isoforms, may be involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to sites of inflammation. CD44v6 containing isoforms may be involved in tumor metastasis.
• Synonyms: CD44, LHR, MDU2, MDU3, MIC4, Phagocytic glycoprotein I, PGP-1, HUTCH-I, Extracellular matrix receptor-III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, CDw44