Tumor necrosis factor (TNF-alpha) Rat Monoclonal Antibody [Clone ID: MP6-XT3]

CAT#: SM112P

Tumor necrosis factor (TNF-alpha) rat monoclonal antibody, clone MP6-XT3, Purified

Product Images

Recombinant Mouse TNF alpha detected in a sandwich assay with Rat anti Mouse TNF alpha (SM112P) as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin as the detetction antibody and Streptavidin:HRP as the final layer.

Specifications

Product Data

• Clone Name: MP6-XT3
• Applications: ELISA, FC, WB
• Recommended Dilution: Western blot.
  ELISA: 5 µg/ml.
  Flow Cytometry: Membrane permeabilisation is required for this application.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant murine TNF-alpha expressed in E. coli.
• Specificity: This antibody is specific for tumour necrosis factor alpha (TNF alpha), also known as TNFSF2. Clone MP6-XT3 is reported to neutralise the effects of mouse TNF alpha. We recommend the use of SM112A for this purpose.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography on Protein G
• Background: This 17kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immunoregulatory role.
• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin