CD49b (ITGA2) Mouse Monoclonal Antibody [Clone ID: 16B4]

CAT#: SM1134P

CD49b (ITGA2) mouse monoclonal antibody, clone 16B4, Purified

Product Images

Staining of Human peripheral blood platelets with Mouse anti Human CD49b antibody.

Specifications

Product Data

• Clone Name: 16B4
• Applications: ELISA, FC, IHC, IP, WB
• Recommended Dilution: ELISA: 10 µg/ml.
  Western Blot.
  Flow Cytometry: 30 µg/ml;
  Immunoprecipitation: 25-50 µg/ml.
  Immunohistochemistry on Frozen Sections: 30-40 µg/ml.
  Immunohistochemistry on Paraffin Sections (requires antigen retrieval using heat treatment prior to staining of paraffin sections).
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified human beta1 preparation from HT1080 fibrosarcoma cell extract.
  Spleen cells from immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.
• Specificity: This antibody is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labelled cell line extract.
  It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha 2
• Database Link:
  Entrez Gene 3673 Human
• Background: CD49b, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin alpha-2 chain. CD49b associates noncovalently with CD29, the integrin beta-1 subunit, to form the heterodimer integrin a2b1 that binds laminin and collagen. It is expressed on B and T lymphocytes, NK cells, monocytes, platelets, fibroblasts and long-term cultured T cells. CD49b/CD29 mediates the Mg++ dependent adhesion of platelets to collagen. It may also be utilized by fibroblasts for the reorganization of the collagen matrix during would healing.
• Synonyms: Integrin alpha-2, ITGA-2, VLA-2 alpha, VLA2, GPIa, Collagen Receptor