CD53 Mouse Monoclonal Antibody [Clone ID: MEM-53]

CAT#: SM1137R

CD53 mouse monoclonal antibody, clone MEM-53, PE

Product Images

Surface staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) PE.

Specifications

Product Data

• Clone Name: MEM-53
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Leukocytes of pacient suffering from a LGL-type leukemia
• Specificity: This antibody reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. It reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
• Formulation: Phosphate buffered saline (PBS) containing 15 mM sodium azide
  Label: PE
  State: Liquid Ig fraction
  Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions
• Purification: Size-exclusion chromatography
• Conjugation: PE
• Storage: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 963 Human
• Background: CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
• Synonyms: Cell surface glycoprotein CD53, Tetraspanin-25, MOX44, TSPAN25