CD55 / DAF Mouse Monoclonal Antibody [Clone ID: 67]

CAT#: SM1141P

CD55 / DAF mouse monoclonal antibody, clone 67, Purified

Product Images

Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD55 (SM1141P).

Specifications

Product Data

• Clone Name: 67
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/10-1/25 diluted antibody to label 10e6 cells in 100 µl.
  Immunohistochemistry on Frozen Sections: 1/100-1/1000.
  Recommended Positive Control Tissue: Human Tonsil.
  (Successful use in immunohistochemistry on paraffin embedded sections (1/50-1/100 using heat treatment for antigen retrieval) was reported by some researchers. As this detection method has not been verified by Acris Antibodies, the application was deleted from the database. This does not necessarily exclude the use in such procedure).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: K562 cells
• Specificity: This antibody recognises the CD55 cell surface antigen.
  Erythrocyte expression is very weak.
  Please Note: We do not recommend the use of this reagent to stain Erythrocytes.
• Formulation: PBS,pH 7.4, with 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Background: CD55 is the complement regulatory protein, decay accelerating factor (DAF). It is a 70kD glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-haemopoietic tissues, particularly epithelium and is specifically found at the foetal-maternal interface in placenta. Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin.
  CD55 is expressed by a wide range of cell types.