CD56 (NCAM1) Mouse Monoclonal Antibody [Clone ID: ERIC-1]

CAT#: SM1142P

CD56 (NCAM1) mouse monoclonal antibody, clone ERIC-1, Purified


USD 475.00

5 Days*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name ERIC-1
Applications ELISA, IHC, WB
Recommended Dilution ELISA: 80 ng/ml.
Immunoblotting.
Immunohistochemistry on Frozen Sections (1/50-1/100).
Recommended Positive Control: Neuroblastoma.
Reactivities Human, Porcine
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Spleen cells from immunised BALB/c mice were fused with cells of the P3/X63.Ag8 mouse myeloma line.
Specificity This antibody recognises N-CAM expressed on developing and adult neuroectodermal tissues.
Neuroectodermal tumours also stain including Glioma, ependymoma, neuroblastoma, medulloblastoma, retinoblastoma and teratoma. Oat cell carcinoma and Wilms tumour are also highly reactive. Will react on Natural Killer cells. Recognises 140, 180 and 120 kD NCAM isoforms.
Formulation PBS containing 0.09% Sodium Azide as preservative.
State: Purified
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Affinity Chromatography on Protein A.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.
Synonyms NCAM-1, N-CAM-1, NCAM
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.