CD56 (NCAM1) Mouse Monoclonal Antibody [Clone ID: ERIC-1]

CAT#: SM1142PT

CD56 (NCAM1) mouse monoclonal antibody, clone ERIC-1, Purified

Specifications

Product Data

• Clone Name: ERIC-1
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 80 ng/ml.
  Immunoblotting.
  Immunohistochemistry on Frozen Sections (1/50-1/100).
  Recommended Positive Control: Neuroblastoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Spleen cells from immunised BALB/c mice were fused with cells of the P3/X63.Ag8 mouse myeloma line.
• Specificity: This antibody recognises N-CAM expressed on developing and adult neuroectodermal tissues.
  Neuroectodermal tumours also stain including Glioma, ependymoma, neuroblastoma, medulloblastoma, retinoblastoma and teratoma. Oat cell carcinoma and Wilms tumour are also highly reactive. Will react on Natural Killer cells. Recognises 140, 180 and 120 kD NCAM isoforms.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 4684 Human
• Background: NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.
• Synonyms: NCAM-1, N-CAM-1, NCAM