CD59 Mouse Monoclonal Antibody [Clone ID: MEM-43]

CAT#: SM1145P

CD59 mouse monoclonal antibody, clone MEM-43, Purified

Product Images

Staining of Human peripheral blood lymphocytes with Mouse anti Human CD59 antibody

Specifications

Product Data

• Clone Name: MEM-43
• Applications: EM, FC, FN, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunoprecipitation.
  Immunoelectron Microscopy .
  Immunofluorescence.
  Western blot (non-reducing conditions).
  Flow Cytometry: Use 10 µl of 1/5-1/10 diluted antibody to label 10⁶ cells in 100 µl.
  Immunohistochemistry on Frozen and Paraffin Sections (Requires antigen retrieval using heat treatment methods prior to staining of paraffin sections; Sodium citrate buffer pH6.0 is recommended for this purpose).
  Positive Control: Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Thymocytes and T lymphocytes
• Specificity: Reacts with a PI-linked glycoprotein, Mw 18-20kD found on all types of leucocytes including platelets. The antigen is expressed on many haemapoietic and non-haemapoietic cells. MEM-43 has been shown to stimulate NK activity.
  CD59 is expressed by erythrocytes and therefore staining in whole blood systems requires a high concentration of antibody.
  The epitope recognized by MEM-43 is lost after reduction, therefore, non-reducing conditions are required for western blotting techniques (2,5).
• Formulation: PBS with 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fration.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Should this product contain a precipitate we recommend microcentrifugation before use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 966 Human
• Background: CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. It is a potent inhibitor of the complement membrane attack complex action. It acts by binding to the C8 and/or C9 complements of the membrane attack complex, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is also involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
• Synonyms: MAC-inhibitory protein, Protectin, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MSK21, MACIF, MAC-IP, MIRL, HRF20, HRF-20