CD62P (SELP) Mouse Monoclonal Antibody [Clone ID: AK-6]

CAT#: SM1150A

CD62P (SELP) mouse monoclonal antibody, clone AK-6, Azide Free

Product Images

Flow Cytometry: CD62P antibody staining of Thrombin activated Human platelets.

Specifications

Product Data

• Clone Name: AK-6
• Applications: FC, IHC, WB
• Recommended Dilution: Western Blot.
  Flow Cytometry: Use 10 µl of 1/10-1/100 diluted antibody to label 10⁶ cells in 100 µl.
  Immunohistochemistry on Frozen Sections: 1/1000-1/5000.
  Immunohistochemistry on Paraffin Sections: 1/500-1/2000. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using Sodium Citrate buffer pH6.0.
  Recommended Positive Control Tissue: Tonsil.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human platelet membrane glycoproteins.
• Specificity: This antibody recognises the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells.
• Formulation: PBS, pH 7.4 without preservatives
  State: Azide Free
  State: Liquid purified IgG fraction
  Stabilizer: None
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store the antibody undiluted at -20°C Only.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: selectin P
• Database Link:
  Entrez Gene 6403 Human
• Background: CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
  The antigen is expressed in the surface of activated platelets and also on endothelial cells both of blood and lymph vessels.
• Synonyms: SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3