CD62P (SELP) Mouse Monoclonal Antibody [Clone ID: AK-6]

CAT#: SM1150F

CD62P (SELP) mouse monoclonal antibody, clone AK-6, FITC

Product Images

Staining of thrombin activated human peripheral blood platelets with Mouse Anti-Human CD62P Antibody FITC conjugated.

Specifications

Product Data

• Clone Name: AK-6
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10⁶ cells in 100 µl.
  We recommend that this antibody be carefully titred against any previous batches to enable correct comparisons to be made with earlier results.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human platelet membrane glycoproteins.
• Specificity: This antibody reacts with a 140 kD antigen expressed on activated platelets and also on endothelial cells both of blood and lymph vessels.
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
  Absorption emission: 490 nm / 525 nm
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: selectin P
• Database Link:
  Entrez Gene 6403 Human
• Background: CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
• Synonyms: SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3