CD100 / SEMA4D Mouse Monoclonal Antibody [Clone ID: A8]

CAT#: SM1174APC

CD100 / SEMA4D mouse monoclonal antibody, clone A8, APC

Product Images

Peripheral Human lymphocytes stained with Mouse Anti Human CD100-APC (SM1174APC).

Specifications

Product Data

• Clone Name: A8
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human PHA activated lymphocytes.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognizes the human CD100 cell surface antigen.
• Formulation: PBS, pH 7.4 containing 5% sucrose, 0.09% Sodium Azide as preservative, 5% Sucrose and 1% BSA as stabilizer.
  Label: APC
  State: Lyophilized purified IgG fraction.
  Label: Allophycocyanin
• Reconstitution Method: Restore with 1 ml distilled water
• Concentration: 0.1 mg/ml
• Conjugation: APC
• Background: Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.
• Synonyms: Semaphorin-4D, SEMAJ, BB18, A8, GR3