TNFRSF1A (full length) Mouse Monoclonal Antibody [Clone ID: H398]

CAT#: SM1185AF

TNFRSF1A (full length) mouse monoclonal antibody, clone H398, Purified

Specifications

Product Data

• Clone Name: H398
• Applications: ELISA, FC, FN, IHC, IP, WB
• Recommended Dilution: Western blot.
  Flow Cytometry.
  Functional assays (antagonistic).
  Immunoassays.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity:
  The monoclonal antibody H398 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor.
  Does not react with TNF-RII.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% BSA
• Concentration: lot specific
• Purification: Protein G Chromatography
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: tumor necrosis factor receptor superfamily member 1A
• Database Link:
  Entrez Gene 7132 Human
• Background: TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways.
• Synonyms: Tumor necrosis factor receptor 1, TNF-R1, TNF-RI, TNFR-I, p55, p60, Tnfrsf1a
• Note: Be aware that the antibody competes with TNF-alpha.