TNFRSF1A (full length) Mouse Monoclonal Antibody [Clone ID: H398]

CAT#: SM1185B

TNFRSF1A (full length) mouse monoclonal antibody, clone H398, Biotin

Specifications

Product Data

• Clone Name: H398
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
  Flow cytometry: The typical starting working dilution is 1:50.
  Immunoassays.
  Western blot: The typical starting working dilution is 1:50.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity:
  The monoclonal antibody H398 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor.
  Does not react with TNF-RII.
• Formulation: PBS
  Label: Biotin
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 1% bovine serum albumin
  Preservative: 0.02 % sodium azide
• Concentration: lot specific
• Purification: Protein G
• Conjugation: Biotin
• Storage:

  Store at 2 - 8 °C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: tumor necrosis factor receptor superfamily member 1A
• Database Link:
  Entrez Gene 7132 Human
• Background:

  TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways.

• Synonyms: Tumor necrosis factor receptor 1, TNF-R1, TNF-RI, TNFR-I, p55, p60, Tnfrsf1a
• Note: Be aware that the antibody competes with TNF-alpha.