TNFRSF1A (full length) Mouse Monoclonal Antibody [Clone ID: H398]

CAT#: SM1185F

TNFRSF1A (full length) mouse monoclonal antibody, clone H398, FITC

Specifications

Product Data

• Clone Name: H398
• Applications: FC
• Recommended Dilution: Flow cytometry (use 10 µl of neat antibody to label 1x10e6 cells or 100 µl whole blood).
  This product is routinely tested in flow cytometry on human peripheral blood monocytes.
• Reactivities: Human, Rabbit
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified human Tumor Necrosis Factor Receptor type 1
• Specificity: H398 recognises an extracellular domain of the 55kD human TNF receptor (p55, TNF-R1, CD120a) which is weakly expressed by monocytes and granulocytes.
  No binding occurs to the 75 kD TNF receptor (CD120b).
• Formulation: PBS, pH 7.4, containing 1% BSA and 0.09% sodium azide
  Label: FITC
  State: Liquid purified IgG fraction
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Protein A affinity chromatography
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Protect from light. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tumor necrosis factor receptor superfamily member 1A
• Database Link:
  Entrez Gene 7132 Human
• Background: Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF Receptor superfamily along with FAS antigen and CD40. TNF Receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 41BB, which are characterized by cysteine rich motifs in the extracellular domain. While TNF Receptor I and TNF Receptor II share 28% sequence homology in the extracellular domains, their intracellular domains lack sequence homology, suggesting that they differ in their internal signal transduction pathways.
  TNF Receptor I contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein), and subsequent interactions with FADD. TNF Receptor I can also activate the transcription factor NFkB via TRAF2 (TNF Receptor associated factor 2). The cytoplasmic domain of TNF Receptor I can directly interact with Jak kinase, thereby activating the JAK/STAT signal transduction cascade.
  TNF Receptor I is expressed by virtually all nucleated mammalian cells, including hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34 + hematopoietic progenitors. Both TNF alpha and TNF beta bind to TNF Receptor I.
• Synonyms: Tumor necrosis factor receptor 1, TNF-R1, TNF-RI, TNFR-I, p55, p60, Tnfrsf1a