CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: LOB 3/17]

CAT#: SM1203FT

CD95 (FAS) mouse monoclonal antibody, clone LOB 3/17, FITC


USD 190.00

5 Days*

Size
    • 25 ug

Product Images

Specifications

Product Data
Clone Name LOB 3/17
Applications FC
Recommended Dilution Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivities Human, Monkey
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Fusion protein comprising extracellular domain of human Fas linked to human Fc.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Specificity This antibody recognises the CD95 cell surface antigen.
Formulation PBS, pH7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Label: FITC
State: Liquid purified IgG fraction.
Label: Fluorescein Isothiocyanate Isomer 1
Concentration lot specific
Purification Affinity Chromatography on Protein A.
Conjugation FITC
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites.
CD95 is expressed by activated T and B cells, NK cells and thymocytes.
Synonyms FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.