Fibroblasts / Epithelial Cells Mouse Monoclonal Antibody [Clone ID: D7-FIB]

CAT#: SM1214R

Fibroblasts / Epithelial Cells mouse monoclonal antibody, clone D7-FIB, PE


USD 620.00

5 Days*

Size
    • 100 Tests

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Specifications

Product Data
Clone Name D7-FIB
Applications FC
Recommended Dilution Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
This product is routinely tested in Flow Cytometry on the KG1 cell line.
Reactivities Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Human foreskin Fibroblasts.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
Specificity This antibody recognizes a ~112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes.
Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.
In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.
D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes. (3). 
Negative Species: Mouse, Rat.
Formulation PBS, pH 7.4
Label: PE
State: Lyophilized purified IgG fraction from Tissue Culture Supernatant
Stabilizer: 1% BSA, 5% Sucrose
Preservative: 0.09% Sodium Azide
Label: R. Phycoerythrin (RPE)
Reconstitution Method Restore with 0.25 ml (SM1214RT) or 1 ml (SM1214R) distilled water.
Purification Affinity Chromatography on Protein G
Conjugation PE
Background A fibroblast is a connective-tissue cell of mesenchymal (somewhat undifferentiated) origin that secretes proteins from which the extracellular fibrillar matrix of connective tissue forms. Epithelial cells are cells that cover the surface of the body and line its cavities.
Synonyms Fibroblast Marker, Fibroblasten
Reference Data

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