HLA Class I ABC Mouse Monoclonal Antibody [Clone ID: W6/32]

CAT#: SM1222R

HLA Class I ABC mouse monoclonal antibody, clone W6/32, PE

Specifications

Product Data

• Clone Name: W6/32
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 μl of neat antibody to label 1x10⁶ cells or 100 μl whole blood.
  This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
• Reactivities: Bovine, Feline, Human, Monkey, Primate
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified human tonsil lymphocyte membranes.
  Spleen cells from immunised BALB/c mice were fused with cells of the NS1/1 - Ag4.1 mouse myeloma cell line.
• Specificity: Antibody SM1222R recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha-3 and alpha-2 domains (See Ishitani, A. et al. for details).
  MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells.
• Formulation: PBS
  Label: PE
  State: Lyophilized purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1ml distilled water
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Background: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
• Synonyms: MHC class I ABC, HLA Class 2 ABC