HLA class I B-27 alpha / HLA-B27 Mouse Monoclonal Antibody [Clone ID: HLA-ABC-m3]

CAT#: SM1305F

HLA class I B-27 alpha / HLA-B27 mouse monoclonal antibody, clone HLA-ABC-m3, FITC

Specifications

Product Data

• Clone Name: HLA-ABC-m3
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat Antibody to label 10⁶ cells or 100 µl of whole blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Immune complex precipitated from an HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal protein A.
• Specificity: This antibody clone HLA-ABC-m3 is a cytotoxic antibody which detects HLA-B27 alloantigen.
  This antibody reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.
  Affinity studies by Scatchard analysis showed that HLA-B27 has a higher affinity for HLA-B27 (9.7 x 10⁸M^-1) than for HLA B7 (9.5 x 10⁷ M^-1).
  Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.
• Formulation: PBS, pH 7.4
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 0.2% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 50 µg/ml
• Purification: Ion Exchange Chromatography
• Conjugation: FITC
• Background: Involved in the presentation of foreign antigens to the immune system.
  HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.
• Synonyms: HLA-B, HLAB, MHC class I antigen B-27