Amylin (IAPP) Mouse Monoclonal Antibody [Clone ID: R10/99]

CAT#: SM1341PT

Amylin (IAPP) mouse monoclonal antibody, clone R10/99, Supernatant

Product Images

Staining of paraffin embedded human pancreas with Mouse anti Human Amylin Peptide (Cat.-No SM1341), showing labeeling of Islet of Langerhans

Specifications

Product Data

• Clone Name: R10/99
• Applications: IF, IHC
• Recommended Dilution: Immunocytochemistry/Immunofluorescence: 1/10-1/500.
  Immunohistochemistry on Frozen Sections: 1/100.
  Immunohistochemistry on Paraffin Sections: 1/100. This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide: CATQRLANFLV coupled to Tuberculin.
  Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2/0 myeloma cell line.
• Specificity: This antibody recognizes the islet Amyloid polypeptide.
  The antibody is of high affinity, and stains islet amyloid peptide in intra-cellular insulin granules, intra-cellular Amyloid and extra-cellular islet Amyloid.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3375 Human
• Background: Amylin is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. While the assosciation of amylin with the development of type II diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Recent results suggest that amylin, like the related beta amyloid (Abeta) assosciated with Alzheimer's disease, can induce apoptotic cell death in particular cultured cells, an effect that may be relevant to the development of type II diabetes.
• Synonyms: AMYLIN; DAP; IAP