Cytokeratin 14 (KRT14) Mouse Monoclonal Antibody [Clone ID: LL002]

CAT#: SM1359

Cytokeratin 14 (KRT14) mouse monoclonal antibody, clone LL002, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with Cytokeratin 14 antibody Cat.-No. SM1359 using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of squamous cells.

Specifications

Product Data

• Clone Name: LL002
• Applications: IHC, WB
• Recommended Dilution: Western blot.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Recommended Positive Control: Skin, tonsil or squamous cell carcinoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of 15 amino acid residues from the C-terminus of Human Cytokeratin 14
• Specificity: LL002 distinguishes stratified epithelial cells from simple epithelial cells and has been reported useful in the identification of squamous cell carcinomas.
  Cellular Localization: Cytoplasmic.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens integrin subunit beta 3 (ITGB3)
• Database Link:
  Entrez Gene 3690 HumanEntrez Gene 3861 Human
• Background: Cytokeratin is a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14.
  Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.
• Synonyms: Cytokeratin-14, CK14, Keratin 14, K14, KRT14

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton