Cytokeratin 14 (KRT14) Mouse Monoclonal Antibody [Clone ID: LL002]

CAT#: SM1359F

Cytokeratin 14 (KRT14) mouse monoclonal antibody, clone LL002, FITC

Specifications

Product Data

• Clone Name: LL002
• Applications: FC, IF
• Recommended Dilution: Immunofluorescence.
  Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10⁶ cells in 100 µl (Requires Membrane Permeabilisation).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Last 15 C-terminal residues of Human cytokeratin 14 conjugated to thyroglobulin.
• Specificity: Recognizes Cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express Cytokeratin 14.
  Mouse anti Cytokeratin 14, clone LL002 has been reported to be suitable for use in Western blotting (Alam et al. 2011).
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3861 Human
• Background: Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.
• Synonyms: Cytokeratin-14, CK14, Keratin 14, K14, KRT14