Factor I (CFI) Mouse Monoclonal Antibody [Clone ID: OX-21]

CAT#: SM1376P

Factor I (CFI) mouse monoclonal antibody, clone OX-21, Purified

Specifications

Product Data

• Clone Name: OX-21
• Applications: ELISA, FC, IHC, IP, R, WB
• Recommended Dilution: ELISA.
  Titre: 1/40 000 (plates coated with 2 µg/ml purified Factor I and HRPO Goat anti-Mouse IgG secondary antibody).
  Western Blot.
  Flow Cytometry.
  Immunoprecipitation.
  Immunohistochemistry on Paraffin Sections (20 µg/ml).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Human Complement Factor 1.
  Spleen cells from immunised FI cross DBA/2 x Balb/c mice were fused with cells of the NS-1 mouse myeloma cell line.
• Specificity: This monoclonal antibody clone OX-21 recognizes Human Complement Protein Factor I.
  Factor I has two polypeptide chains, 56kD and 38kD. This antibody detects only the 56kD chain.
  This clone has been reported not to cross react with Rat.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Protein G Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens complement factor I (CFI), transcript variant 2
• Database Link:
  Entrez Gene 3426 Human
• Background: Factor I is a plasma protein of molecular weight 88 kDa, consisting of two disulphide linked chains of 50 and 38 kDa. Factor I is a serine protease with a high degree of specificity for C3b and C4b. It has a requirement for protein cofactors for cleavage of these complement proteins, Factor H, CR1 or MCP are required for C3b cleavage and C4bp or CR1 for C4b cleavage.
• Synonyms: CFI, IF, KAF, AHUS3, C3BINA, C3b-INA

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades