FITC Rat Monoclonal Antibody [Clone ID: LO-FLUO-1]

CAT#: SM1474B

FITC rat monoclonal antibody, clone LO-FLUO-1, Biotin


USD 630.00

5 Days*

Size
    • 500 ug

Product Images

Specifications

Product Data
Clone Name LO-FLUO-1
Applications ELISA, FC
Recommended Dilution ELISA: 0.5 µg/ml.
Flow Cytometry.
Host Rat
Isotype IgM
Clonality Monoclonal
Immunogen FITC conjugated Mouse immunoglobulin.
Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
Specificity This antibody reacts with Fluorescein Isothiocyanate.
Avidity against FITC conjugated BSA is 2 x 10e10M-1.
This antibody binds to both Free FITC and FITC conjugated to proteins, and may be valuable for amplification of FITC based immunostaining.
Formulation PBS containing 50% Glycerol and 0.09% Sodium Azide
Label: Biotin
State: Liquid purified IgM fraction.
Concentration 1.0 mg/ml
Purification Affinity Chromatography.
Conjugation Biotin
Background FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
Synonyms Fluorescein Isothiocyanate
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.