Myeloperoxidase (MPO) Mouse Monoclonal Antibody [Clone ID: 2C7]

CAT#: SM1475F

Myeloperoxidase (MPO) mouse monoclonal antibody, clone 2C7, FITC

Product Images

Staining of permeabilised human peripheral blood granulocytes with Mouse anti Human Myeloperoxidase Antibody -FITC

Specifications

Product Data

• Clone Name: 2C7
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10⁶ cells in 100 µl.
  Membrane permeabilisation is required for this application.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human myeloperoxidase.
  Spleen cells from immunised mice were fused with cells of the mouse X63 AG8-653 myeloma cell line.
• Specificity: This antibody recognises myeloperoxidase (MPO), an important component of azurophilic granules in neutrophils.
  Negative Species: Rat.
• Formulation: PBS, pH 7.4
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: myeloperoxidase
• Database Link:
  Entrez Gene 4353 Human
• Background: Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.
• Synonyms: MPO