Myeloperoxidase (MPO) Mouse Monoclonal Antibody [Clone ID: 2C7]

CAT#: SM1475R

Myeloperoxidase (MPO) mouse monoclonal antibody, clone 2C7, PE

Specifications

Product Data

• Clone Name: 2C7
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl (Membrane permeabilisation is required).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human Myeloperoxiase.
  Spleen cells from immunised mice were fused with cells of the mouse X63 AG8-653 myeloma cell line.
• Specificity: This antibody recognizes Myeloperoxidase (MPO).
  It may be used for the Flow Cytometric detection of Myeloid cells following membrane permeablisation.
  Negative Species: Rat.
• Formulation: PBS
  Label: PE
  State: Lyophilized purified IgG fraction
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1 ml distilled water
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Storage: Store undiluted Prior to and following reconstitution at 2-8°C.
  DO NOT FREEZE!
  This antibody is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: myeloperoxidase
• Database Link:
  Entrez Gene 4353 Human
• Background: MPO is an important component of azurophilic granules in neutrophils.
  Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 - 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.
• Synonyms: MPO