CD19 Mouse Monoclonal Antibody [Clone ID: LT19]

CAT#: SM1529P

CD19 mouse monoclonal antibody, clone LT19, Purified

Specifications

Product Data

• Clone Name: LT19
• Applications: FC, IHC, IP
• Recommended Dilution: Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: The antibody LT19 reacts with CD19 (B4), a 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction from Ascites
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Ion Exchange Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 930 Human
• Background: CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
• Synonyms: Leu-12, B-cell marker