CMA1 Mouse Monoclonal Antibody [Clone ID: CC1]

CAT#: SM1536P

CMA1 mouse monoclonal antibody, clone CC1, Purified

Specifications

Product Data

• Clone Name: CC1
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/500-1/1000.
  Does not require protein digestion pre-treatment of paraffin sections.
  Does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
  Recommended Positive Control: Skin.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Human skin chymase.
  Spleen cells from immunised BALB/c mice were fused with cells of the P3/NSI/I.Ag4.1 mouse myeloma cell line.
• Specificity: This antibody binds to mast cell chymase, it may be used to detect mast cells in routinely fixed tissues, and has been used to study the distribution of mast cells in skin, synovium, lung and heart.
  Binding of this antibody to chymase is not reduced in the presence of heparin, suggesting that the antibody does not bind to the heparin-binding domain of chymase.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1215 Human
• Background: Mast Cell Chymase is an important marker of mast cells and also an important mediator of inflammation.
• Synonyms: Alpha-chymase, Mast cell protease I, CMA1, CYH, CYM