Cd68 Rat Monoclonal Antibody [Clone ID: FA-11]

CAT#: SM1550B

Cd68 rat monoclonal antibody, clone FA-11, Biotin


USD 505.00

5 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name FA-11
Applications FC
Recommended Dilution Flow Cytometry: Use 10 µl of Neat-1/10 dilueted antibody to label 106 cells in 100 µl.
Membrane permeabilisation is required for this application. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Reactivities Mouse
Host Rat
Isotype IgG2a
Clonality Monoclonal
Immunogen Purified Concanavalin A acceptor glycoprotein from P815 cell line
Specificity This CD68 antibody clone FA-11 does detect surface Macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Formulation PBS, pH 7.4
Label: Biotin
State: Liquid purified IgG fraction from Tissue Culture Supernatant
Stabilizer: 1% BSA
Preservative: 0.09% Sodium Azide
Concentration 0.1 mg/ml
Purification Affinity Chromatography on Protein G
Conjugation Biotin
Background The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.
Synonyms Gp110, Macrosialin, Macrophage marker
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.