Cd68 Rat Monoclonal Antibody [Clone ID: FA-11]

CAT#: SM1550R

Cd68 rat monoclonal antibody, clone FA-11, PE


USD 620.00

5 Days*

Size
    • 100 Tests

Product Images

Specifications

Product Data
Clone Name FA-11
Applications FC
Recommended Dilution Flow Cytometry: Use 10 µl of neat antibody to label 106 cells in 100 µl (Membrane permeabilisation is required) . 
Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin-embedded tissues ( Masaki et al. 2003) and Devey et al. 2009). 
Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Reactivities Mouse
Host Rat
Isotype IgG2a
Clonality Monoclonal
Immunogen Purified Concanavalin A acceptor glycoprotein from P815 cell line.
Specificity The antibody recognizes CD68.
SM1550R does detect surface Macrosialin at low levels in resident Mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Formulation PBS
Label: PE
State: Lyophilized purified IgG fraction from tissue culture supernatant
Stabilizer: 1% BSA, 5% Sucrose
Preservative: 0.09% Sodium Azide
Label: R. Phycoerythrin (RPE)
Reconstitution Method Restore with 1.0 ml distilled water.
Purification Affinity Chromatography on Protein G
Conjugation PE
Background Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.
Synonyms Gp110, Macrosialin, Macrophage marker
Reference Data

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