Cd34 Rat Monoclonal Antibody [Clone ID: MEC14.7]

CAT#: SM1603LE

Cd34 rat monoclonal antibody, clone MEC14.7, Low Endotoxin

Specifications

Product Data

• Clone Name: MEC14.7
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry.
  Immunoprecipitation.
  Immunohistochemistry on Paraffin Sections: 1/20-1/200.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody recognises the CD34 cell surface antigen, wihich is expressed by endothelial cells and by haematopoietic stem cells. It recognises a Neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity.
  Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.
• Formulation: PBS without preservatives.
  State: Low Endotoxin
  State: Liquid purified IgG fraction.
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G from tissue culture supernatant.
• Database Link:
  Entrez Gene 12490 Mouse
• Background: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
• Synonyms: Hematopoietic progenitor cell marker
• Note: Endotoxin Level: less than 0.01 EU/µg