TLR4 Mouse Monoclonal Antibody [Clone ID: HTA125]

CAT#: SM1677B

TLR4 mouse monoclonal antibody, clone HTA125, Biotin

Specifications

Product Data

• Clone Name: HTA125
• Applications: FC
• Recommended Dilution: Flow cytometry.
• Reactivities: Canine, Guinea Pig, Human, Monkey, Porcine
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
• Specificity: This antibody recognises the Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of SM1677LE for this purpose.
• Formulation: PBS containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: Biotin
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 7099 Human
• Background: TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
• Synonyms: Toll-like receptor 4