TLR4 Mouse Monoclonal Antibody [Clone ID: HTA125]

CAT#: SM1677R

TLR4 mouse monoclonal antibody, clone HTA125, PE

Product Images

Staining of U937 cells with RPE conjugated Mouse Anti Human CD284 Antibody PE (Cat.-No SM1677R).

Specifications

Product Data

• Clone Name: HTA125
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10⁶ cells or 100 µl whole blood.
• Reactivities: Canine, Guinea Pig, Human, Monkey, Porcine
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Ba/F3 cell line expressing TLR4 (CD284).
  Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
• Specificity: This antibody recognises the Toll like receptor 4 (TLR4) cell surface antigen.
  This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of SM1677LE for this purpose.
• Formulation: PBS
  Label: PE
  State: Lyophilized purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: 1% BSA and 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1.0 ml distilled water.
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Storage:

  Prior to and following reconstitution store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 7099 Human
• Background: TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
• Synonyms: Toll-like receptor 4