Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse Monoclonal Antibody [Clone ID: SV5-PK1]

CAT#: SM1691

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) mouse monoclonal antibody, clone SV5-PK1, Purified

Product Images

V5 tagged protein detected with Mouse anti V5-Tag (SM1691)

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  V5 tagged protein detected with Mouse anti V5-Tag (SM1691)

Specifications

Product Data

• Clone Name: SV5-PK1
• Applications: ELISA, FC, IF, IHC, IP, R, WB
• Recommended Dilution: ELISA: 1/1000-1/5000.
  Western Blot: 1/1000-1/5000.
  Immunoprecipitation.
  Radioimmunoassays.
  Immunofluorescence.
  Flow Cytometry.
  Immunohistochemistry on Frozen Sections.
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Paramyxovirus Simian-Virus 5 (SV5)
• Specificity: Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labelled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005).
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Background: The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).