Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse Monoclonal Antibody [Clone ID: SV5-PK1]

CAT#: SM1691F

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) mouse monoclonal antibody, clone SV5-PK1, FITC

Specifications

Product Data

• Clone Name: SV5-PK1
• Applications: IF
• Recommended Dilution: Immunofluorescence: 1/100; Membrane permeabilisation with 0.5% IGEPAL is required for this application.
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
• Specificity: This antibody recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. This antibody has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr.
• Formulation: Containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Note: This product is manufactured under an exclusive license from the University of St. Andrews,UK.