Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse Monoclonal Antibody [Clone ID: SV5-PK1]

CAT#: SM1691HRP

Pk (V5) Epitope Tag (GKPIPNPLLGLDST) mouse monoclonal antibody, clone SV5-PK1, HRP

Specifications

Product Data

• Clone Name: SV5-PK1
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western Blot (1/1000).
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mice were infected with the paramyxovirus SV5, Simian-Virus 5.
  Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
• Specificity: This antibody recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
  This antibody has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used.
  The 14 amino acid epitope is: gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).
• Formulation: Label: HRP
  State: Liquid purified IgG fraction containing 0.01% Thiomersal as preservative.
  Label: Horseradish Peroxidase
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Conjugation: HRP